The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species.

BACKGROUND: Two endemic lycophyte species Isoetes cangae and Isoetes serracarajensis have been recently described in the State of Pará in the Amazon forest located in northern Brazil. Isoetes L. has survived through three mass extinctions. Plants are considered small-sized, heterosporous, and can display a great diversity of physiological adaptations to different environments. Thus, the current study aimed to estimate the genetic variation of the populations of I. cangae and I. serracarajensis to generate information about their different mechanisms for survival at the same geographical location that could point to different reproductive, adaptative and dispersal strategies and should be considered for effective conservation strategies. METHODS: The genetic diversity and population structure of I. cangae and I. serracarajensis were investigated using Inter Simple Sequence Repeat (ISSR) molecular markers. Total genomic DNA was isolated, and the genetic diversity parameters were calculated. RESULTS: The sixteen primers produced 115 reproducible bands, 87% of which were polymorphic. A high level of polymorphic loci (81.74% and 68.48%) and a high Shannon index (Sh = 0.376 and 0.289) were observed for I. cangae and I. serracarajensis, respectively. The coefficient of genetic differentiation between population areas (GST) showed a higher value in I. serracarajensis (0.5440). Gene flow was higher in I. cangae (1.715) and lower in I. serracarajensis populations (0.419). Overall, the results further show that I. serracarajensis and I. cangae are two species with considerable genetic variation and that these differences may reflect their habitats and modes of reproduction. These results should be considered in the development of effective conservation strategies for both species.

Recycling of wastes from fish beneficiation by composting: chemical characteristics of the compost and efficiency of their humic acids in stimulating the growth of lettuce.

Waste from the beneficiation of fish was composted with crushed grass aiming to characterize their chemical composition and investigate the possibility of the use of the final compost as source of humic acids (HA) able to stimulate the growth of lettuce. Compost presented pH value, C/N ratio, and electrical conductivity that allow its use as an organic fertilizer. The element content was present in the following order of abundance in the compost: P > Ca > N > Mg > K > Fe > Zn > Mn > Mo > Cu, and the humus composition was similar to that observed in others kind of organic residues composted. The high content of oxygen pointed out a high level of oxidation of HA, in line with the predominance of phenolic acidity in the functional groups. The 13C-NMR spectra showed marked resonances due to the presence of lipids and other materials resistant to degradation as methoxy substituent and N-alkyl groups. A concentration of 20 mg L-1 HA increased significantly both dry and wet root matter in lettuce but the CO2 assimilation, stomatal conductance, and number of lateral roots of the plants were not affected. However, increases of 64% in the water-use efficiency was observed due to the HA addition, probably related to the root morphology alteration which resulted in 1.6-fold increase of lateral root average length and due to the higher H+ extrusion activity. Reuse of residues from the fish beneficiation activity by composting may represent a safe tool to increase the value of recycled organic residues and generate HA with potential use as plant growth stimulants.

Microbiological monitoring in two areas with different levels of conservation in the mangroves of an Ecological Station, Vitoria, ES / Monitoramento microbiológico em duas áreas com diferentes níveis de conservação nos manguezais de uma Estação Ecológica, Vitória, ES

Mangroves are classified as permanent preservation areas and regarded as natural nurseries. However, they have suffered several anthropogenic stresses, resulting in their decline. In the light of that, comes the importance of researching their environmental characteristics and revealing possible factors that have led to the degradation of this important ecosystem. The aim of this study was to evaluate the environmental quality of different areas in the mangroves of Ilha do Lameirão Ecological Station through microbiological analyzes of sediment and interstitial water along ten (10) sites, distributed in two areas with different conservation levels (Canal dos Escravos (CE) and Maria Ortiz (MO)) between 2010 and 2012. The microbiological analyzes revealed that MO region, in all seasons of the year, achieved total coliform and thermo -tolerant coliform values above those permitted by the CONAMA Resolution 357/05, fitting the Class 2 conservation standard. The presence of high levels of total and thermo-tolerant coliforms in MO is a strong indicator of impacts originated from the human population and, consequently, the decline of the mangrove itself and the health of human communities surrounding that area.

Os manguezais são classificados como áreas de preservação permanente e considerados como berçários naturais, no entanto sofrem várias tensões antropogênicas, resultando em seu declínio. À luz disso, evidencia-se a importância de pesquisar suas características ambientais e reveladoras de possíveis fatores que levaram à degradação desse importante ecossistema. O objetivo deste estudo é avaliar a qualidade ambiental das diferentes áreas em manguezais da Estação Ecológica Ilha do Lameirão por meio de análises microbiológicas de sedimentos e da água intersticial ao longo de dez locais, distribuídos em duas áreas com diferentes níveis de conservação (Canal dos Escravos (CE) e Maria Ortiz (MO)) entre 2010 e 2012. As análises microbiológicas revelaram que a região MO, em todas as estações do ano, obteve valores de coliformes termo-tolerantes e totais acima do permitido pela resolução CONAMA 357/05, Classe 2. A presença de níveis elevados de coliformes totais e termo-tolerantes em MO é um forte indicador dos impactos originados da população humana e, consequentemente, do declínio do próprio mangue e da saúde das comunidades humanas que cercam a área.

Indolacetic and humic acids induce lateral root development through a concerted plasmalemma and tonoplast H+ pumps activation.

Increasing evidences have indicated that humic substances can induce plant growth and productivity by functioning as an environmental source of auxinic activity. Here we comparatively evaluate the effects of indole-3-acetic acid (IAA) and humic acids (HA) isolated from two different soils (Inseptsol and Ultisol) and two different organic residues (vermicompost and sewage sludge) on root development and on activities of plasmalemma and tonoplast H(+ )pumps from maize roots. The data show that HA isolated from these different sources as well as low IAA concentrations (10(-10) and 10(-15) M) improve root growth through a markedly proliferation of lateral roots along with a differential activation not only of the plasmalemma but also of vacuolar H(+)-ATPases and H(+)-pyrophosphatase. Further, the vacuolar H(+)-ATPase had a peak of stimulation in a range from 10(-8) to 10(-10) M IAA, whereas the H(+)-pyrophosphatase was sensitive to a much broader range of IAA concentrations from 10(-3) to 10(-15) M. It is proposed a complementary view of the acid growth mechanism in which a concerted activation of the plasmalemma and tonoplast H(+ )pumps plays a key role in the root cell expansion process driven by environment-derived molecules endowed with auxinic activity, such as that of humic substances.